Corn Adventitious Presence (GMO)

Corn Services

Flour ready for testing

Method

This test is intended to test conventional seed for the absence of herbicide and insect traits. Herbicide bioassays are used to screen a large number of seeds (seed needs to be viable) and keep cost low. Insect traits are screened using an ELISA technique. Number of seed tested is typically 1200 or 2400 seed.

AP- Seed- Percentage of trait tolerant seedlings are reported for herbicide bioassays. Insect traits (Mon88017, Mon810, B+11, TC1507, MR162, DAS59122-7, Mon 89034) aare tested for the proteins produced from the transgenic event in 3 pools of 400 of 800 ground seed for a total of 1200 or 2400 seeds.

AP- Grain – Kernels are grouped into “pools” (800 kernels typically), ground into flour, proteins extracted and tested with ELISA kit for proteins produced by the transgenic event, including Glyphosphate and Liberty herbicide proteins.

PCR Testing – Seed are grouped into "pools" (3000 seed typically). DNA is extracted from ground material and tested for the presence of standard DNA sequences utilized with transgenic insertion.

What is Reported

AP- Seed- An estimated purity is reported based on positive responses within "pools" and use of the statistical analysis program "Seedcalc".

AP- Grain - Based on the number of "pools" with positive or negative results and the total number of seed tested, an estimate is generated of the purity of the submitted sample.

PCR Testing - Based on the number of "pools" with positive or negative results and the total number of seed tested, an estimate is generated of the purity of the submitted sample.